Determinants of sequence-specificity within human AID and APOBEC3G
نویسندگان
چکیده
منابع مشابه
Sequence specificity of human telomerase.
DNA replication machinery copies an entire genome with remarkable speed and accuracy in preparation for each cell division. However, DNA polymerase unidirectionality and requirement for a free 3′ hydroxyl group render these enzymes incapable of duplicating the extreme ends of eukaryotic linear chromosomes. This deficiency leads to a gradual loss of terminal sequence with each round of replicati...
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Activation-induced (cytidine) deaminase (AID) efficiently introduces multiple and diversified deaminations in immunoglobulin (Ig) variable and switch regions. Here, we review studies of AID, and the APOBEC family member, APOBEC3G, demonstrating that both enzymes introduce multiple deaminations by processive action on single-stranded DNA and that deaminations occur stochastically at hot- and col...
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Residues responsible for allostery, cooperativity, and other subtle but functionally important interactions remain difficult to detect. To aid such detection, we employ statistical inference based on the assumption that residues distinguishing a protein subgroup from evolutionarily divergent subgroups often constitute an interacting functional network. We identify such networks with the aid of ...
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Abstract Background and Objective: Culture, microscopic method is a gold standard method for identification of Lishmania parasite. The use of Molecular methods such as RT- PCR compared to microscopic methods has a higher sensitivity and specificity however, it is not widely used due to its expensive equipment and the time requested. The use of nucleic acid sequence based amplification (NASBA) ...
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The AID/APOBEC family of enzymes in higher vertebrates converts cytosines in DNA or RNA to uracil. They play a role in antibody maturation and innate immunity against viruses, and have also been implicated in the demethylation of DNA during early embryogenesis. This is based in part on reported ability of activation-induced deaminase (AID) to deaminate 5-methylcytosines (5mC) to thymine. We hav...
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ژورنال
عنوان ژورنال: DNA Repair
سال: 2010
ISSN: 1568-7864
DOI: 10.1016/j.dnarep.2010.02.010